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Phần 1: Nghiên cứu phân tích phân tử về định loài giun lươn gây bệnh ở người

            Giun lươn Strongyloides spp. gây bệnh cho người có thể xuất phát từ 3 loài trong giống Strongyloides, sự phân bố ưu thế từng loài tùy thuộc vào vùng địa lý, sự di chuyển dân di biến động và nhóm dân tỵ nạn, hay các bệnh viện tâm thần và nơi điều trị các bệnh nhân suy giảm miễn dịch đã phân tích cho thấy tỷ lệ từng loài của mỗi nơi cũng khác nhau.

Trên thế giới cũng như tại Việt Nam, một số loài giun lươn đã được phân tích, song chưa được bài bản và quy mô rộng trên các quần thể ở các vùng địa lý khác nhau. Dưới đây là một số nghiên cứu trên thế giới và Việt Nam đã định loài, phân tích phả hệ, di truyền của loài giun lươn bằng nhiều phương pháp và kỹ thuật khác nhau như PCR, Real-time PCR, Nested PCR, LAMP, DNA isolation, RFLP . Xin chia sẻ cùng đồng nghiệp.

A PCR-Based Molecular Detection of Strongyloides stercoralisin Human Stool Samples from Tabriz City, Iran.

Ghasemikhah R, Tabatabaiefar MA, Shariatzadeh SA, Shahbazi A, Hazratian T.

Sci Pharm. 2017 Mar 27;85(2). pii: E17. doi: 10.3390/scipharm85020017.

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Molecular Diagnosis of Strongyloides stercoralis Infection by PCR Detection of Specific DNA in Human Stool Samples.

Moghaddassani H, Mirhendi H, Hosseini M, Rokni M, Mowlavi G, Kia E.

Iran J Parasitol. 2011 Jun;6(2):23-30.

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An improved DNA isolation technique for PCR detection of Strongyloides stercoralis in stool samples.

Repetto SA, Alba Soto CD, Cazorla SI, Tayeldin ML, Cuello S, Lasala MB, Tekiel VS, González Cappa SM.

Acta Trop. 2013 May;126(2):110-4. doi: 10.1016/j.actatropica.2013.02.003. Epub 2013 Feb 12.

PMID: 23416126 Similar articles

Real-time PCR for detection of Strongyloides stercoralis in human stool samples from Côte d'Ivoire: diagnostic accuracy, inter-laboratory comparison and patterns of hookworm co-infection.

Becker SL, Piraisoody N, Kramme S, Marti H, Silué KD, Panning M, Nickel B, Kern WV, Herrmann M, Hatz CF, N'Goran EK, Utzinger J, von Müller L.

Acta Trop. 2015 Oct;150:210-7. doi: 10.1016/j.actatropica.2015.07.019. Epub 2015 Jul 26.

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Strong-LAMP: A LAMP Assay for Strongyloides spp. Detection in Stool and Urine Samples. Towards the Diagnosis of Human Strongyloidiasis Starting from a Rodent Model.

Fernández-Soto P, Sánchez-Hernández A, Gandasegui J, Bajo Santos C, López-Abán J, Saugar JM, Rodríguez E, Vicente B, Muro A.

PLoS Negl Trop Dis. 2016 Jul 14;10(7):e0004836. doi: 10.1371/journal.pntd.0004836. eCollection 2016 Jul.

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Evaluation of techniques for the diagnosis of Strongyloides stercoralis in human immunodeficiency virus (HIV) positive and HIV negative individuals in the city of Itajaí, Brazil.

Marchi Blatt J, Cantos GA.

Braz J Infect Dis. 2003 Dec;7(6):402-8.

PMID: 14636480 Free Article Similar articles

Molecular diagnosis of strongyloidiasis in a population of an endemic area through nested-PCR.

Sharifdini M, Keyhani A, Eshraghian MR, Beigom Kia E.

Gastroenterol Hepatol Bed Bench. 2018 Winter;11(1):68-74.

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Patterns of detection of Strongyloides stercoralis in stool specimens: implications for diagnosis and clinical trials.

Dreyer G, Fernandes-Silva E, Alves S, Rocha A, Albuquerque R, Addiss D.

J Clin Microbiol. 1996 Oct;34(10):2569-71.

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Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction with Parasitological Methods for Detection of Strongyloides stercoralis in Human Fecal Samples.

Sharifdini M, Mirhendi H, Ashrafi K, Hosseini M, Mohebali M, Khodadadi H, Kia EB.

Am J Trop Med Hyg. 2015 Dec;93(6):1285-91. doi: 10.4269/ajtmh.15-0309. Epub 2015 Sep 8.

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Cryptosporidium and Strongyloides stercoralis infections among people with and without HIV infection and efficiency of diagnostic methods for Strongyloides in Yirgalem Hospital, southern Ethiopia.

Getaneh A, Medhin G, Shimelis T.

BMC Res Notes. 2010 Apr 1;3:90. doi: 10.1186/1756-0500-3-90.

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Evaluation of Two DNA Extraction Methods for Detection of Strongyloides stercoralis Infection.

Barda B, Wampfler R, Sayasone S, Phongluxa K, Xayavong S, Keoduangsy K, Schindler C, Keiser J.

J Clin Microbiol. 2018 Mar 26;56(4). pii: e01941-17. doi: 10.1128/JCM.01941-17. Print 2018 Apr.

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Application of real-time PCR for the detection of Strongyloides spp. in clinical samples in a reference center in Spain.

Saugar JM, Merino FJ, Martín-Rabadán P, Fernández-Soto P, Ortega S, Gárate T, Rodríguez E.

Acta Trop. 2015 Feb;142:20-5. doi: 10.1016/j.actatropica.2014.10.020. Epub 2014 Nov 4.

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Conventional PCR for molecular diagnosis of human strongyloidiasis.

Sitta RB, Malta FM, Pinho JR, Chieffi PP, Gryschek RC, Paula FM.

Parasitology. 2014 Apr;141(5):716-21. doi: 10.1017/S0031182013002035. Epub 2014 Jan 28.

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DIAGNOSIS OF Strongyloides stercoralis INFECTION IN IMMUNOCOMPROMISED PATIENTS BY SEROLOGICAL AND MOLECULAR METHODS.

Paula FM, Malta FM, Corral MA, Marques PD, Gottardi M, Meisel DM, Yamashiro J, Pinho JR, Castilho VL, Gonçalves EM, Gryschek RC, Chieffi PP.

Rev Inst Med Trop Sao Paulo. 2016 Sep 22;58:63. doi: 10.1590/S1678-9946201658063.

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Detection of Strongyloides stercoralis infection among cancer patients in a major hospital in Kelantan, Malaysia.

Zueter AM, Mohamed Z, Abdullah AD, Mohamad N, Arifin N, Othman N, Noordin R.

Singapore Med J. 2014 Jul;55(7):367-71.

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Comparison between PCR and larvae visualization methods for diagnosis of Strongyloides stercoralis out of endemic area: A proposed algorithm.

Repetto SA, Ruybal P, Solana ME, López C, Berini CA, Alba Soto CD, Cappa SM.

Acta Trop. 2016 May;157:169-77. doi: 10.1016/j.actatropica.2016.02.004. Epub 2016 Feb 8.

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Hình 1

Molecular diagnosis of Strongyloides stercoralis among transplant candidates.

Paula FM, Malta FM, Marques PD, Melo GB, Corral MA, Gottardi M, Pinho JRR, Gonçalves EMN, Castilho VLP, Pierrotti LC, Abdala E, Costa SF, Chieffi PP, Gryschek RCB.

Transpl Infect Dis. 2018 Apr 20:e12909. doi: 10.1111/tid.12909. [Epub ahead of print]

PMID: 29679429 Similar articles

[Immunodiagnosis of Strongyloides stercoralis infections in Chile using ELISA test].

Mercado R, Jercic MI, Torres P, Alcayaga S, Martins de Paula F, Costa-Cruz JM, Ueta MT.

Rev Med Chil. 2002 Dec;130(12):1358-64. Spanish.

PMID: 12611236 Similar articles

Efficacy of parasitological methods for the diagnosis of Strongyloides stercoralis and hookworm in faecal specimens.

Inês Ede J, Souza JN, Santos RC, Souza ES, Santos FL, Silva ML, Silva MP, Teixeira MC, Soares NM.

Acta Trop. 2011 Dec;120(3):206-10. doi: 10.1016/j.actatropica.2011.08.010. Epub 2011 Aug 27.

PMID: 21896267 Similar articles

Molecular differences between several species of Strongyloides and comparison of selected isolates of S. stercoralis using a polymerase chain reaction-linked restriction fragment length polymorphism approach.

Ramachandran S, Gam AA, Neva FA.

Am J Trop Med Hyg. 1997 Jan;56(1):61-5.

PMID: 9063363 Similar articles


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Diagnosis, treatment and risk factors of Strongyloides stercoralis in schoolchildren in Cambodia.

Khieu V, Schär F, Marti H, Sayasone S, Duong S, Muth S, Odermatt P.

PLoS Negl Trop Dis. 2013;7(2):e2035. doi: 10.1371/journal.pntd.0002035. Epub 2013 Feb 7.

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Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR.

Verweij JJ, Canales M, Polman K, Ziem J, Brienen EA, Polderman AM, van Lieshout L.

Trans R Soc Trop Med Hyg. 2009 Apr;103(4):342-6. doi: 10.1016/j.trstmh.2008.12.001. Epub 2009 Feb 4. Erratum in: Trans R Soc Trop Med Hyg. 2010 May;104(5):378.

PMID: 19195671 Similar articles

Strongyloides stercoralis hyperinfection in a patient with rheumatoid arthritis and bronchial asthma: a case report.

Altintop L, Cakar B, Hokelek M, Bektas A, Yildiz L, Karaoglanoglu M.

Ann Clin Microbiol Antimicrob. 2010 Sep 20;9:27. doi: 10.1186/1476-0711-9-27.

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Real-time polymerase chain reaction for detection of Strongyloides stercoralis in stool.

Sultana Y, Jeoffreys N, Watts MR, Gilbert GL, Lee R.

Am J Trop Med Hyg. 2013 Jun;88(6):1048-51. doi: 10.4269/ajtmh.12-0437. Epub 2013 Apr 8.

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Comorbid gastric adenocarcinoma and gastric and duodenal Strongyloides stercoralis infection: a case report.

Seo AN, Goo YK, Chung DI, Hong Y, Kwon O, Bae HI.

Korean J Parasitol. 2015 Feb;53(1):95-9. doi: 10.3347/kjp.2015.53.1.95. Epub 2015 Feb 27.

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ELISA-based coproantigen in human strongyloidiaisis: a diagnostic method correlating with worm burden.

El-Badry AA.

J Egypt Soc Parasitol. 2009 Dec;39(3):757-68.

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Increased detection rate of Strongyloides stercoralis by repeated stool examinations using the agar plate culture method.

Hirata T, Nakamura H, Kinjo N, Hokama A, Kinjo F, Yamane N, Fujita J.

Am J Trop Med Hyg. 2007 Oct;77(4):683-4.

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Strongyloides stercoralis age-1: a potential regulator of infective larval development in a parasitic nematode.

Stoltzfus JD, Massey HC Jr, Nolan TJ, Griffith SD, Lok JB.

PLoS o­ne. 2012;7(6):e38587. doi: 10.1371/journal.pone.0038587. Epub 2012 Jun 6.

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Diagnostic accuracy of Kato-Katz, FLOTAC, Baermann, and PCR methods for the detection of light-intensity hookworm and Strongyloides stercoralis infections in Tanzania.

Knopp S, Salim N, Schindler T, Karagiannis Voules DA, Rothen J, Lweno O, Mohammed AS, Singo R, Benninghoff M, Nsojo AA, Genton B, Daubenberger C.

Am J Trop Med Hyg. 2014 Mar;90(3):535-45. doi: 10.4269/ajtmh.13-0268. Epub 2014 Jan 20.

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Evaluation of Parasitological Methods for the Detection of Strongyloides Stercoralis among Individuals in Selected Health Institutions In Addis Ababa, Ethiopia.

Hailegebriel T, Petros B, Endeshaw T.

Ethiop J Health Sci. 2017 Sep;27(5):515-522.

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An evaluation of the agar plate method for the detection of Strongyloides stercoralis in northern Thailand.

Koga K, Kasuya S, Khamboonruang C, Sukavat K, Nakamura Y, Tani S, Ieda M, Tomita K, Tomita S, Hattan N, et al.

J Trop Med Hyg. 1990 Jun;93(3):183-8.

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Risk factors for acquiring Strongyloides stercoralis infection among patients attending a tertiary hospital in south India.

Chordia P, Christopher S, Abraham OC, Muliyil J, Kang G, Ajjampur Ss.

Indian J Med Microbiol. 2011 Apr-Jun;29(2):147-51. doi: 10.4103/0255-0857.81797.

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Immunoepidemiologic studies of Strongyloides stercoralis and human T lymphotropic virus type I infections in Jamaica.

Robinson RD, Lindo JF, Neva FA, Gam AA, Vogel P, Terry SI, Cooper ES.

J Infect Dis. 1994 Mar;169(3):692-6.

PMID: 8158055 Similar articles

Parasitological and serological diagnosis of Strongyloides stercoralis in domesticated dogs from southeastern Brazil.

Júnior AF, Gonçalves-Pires MR, Silva DA, Gonçalves AL, Costa-Cruz JM.

Vet Parasitol. 2006 Mar 15;136(2):137-45.

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Strongyloides stercoralis: there but not seen.

Montes M, Sawhney C, Barros N.

Curr Opin Infect Dis. 2010 Oct;23(5):500-4. doi: 10.1097/QCO.0b013e32833df718. Review.

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The prevalence and response to therapy of Strongyloides stercoralis in patients with asthma from endemic areas.

Wehner JH, Kirsch CM, Kagawa FT, Jensen WA, Campagna AC, Wilson M.

Chest. 1994 Sep;106(3):762-6.

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Development of a rapid serological assay for the diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology.

Pak BJ, Vasquez-Camargo F, Kalinichenko E, Chiodini PL, Nutman TB, Tanowitz HB, McAuliffe I, Wilkins P, Smith PT, Ward BJ, Libman MD, Ndao M.

PLoS Negl Trop Dis. 2014 Aug 7;8(8):e3002. doi: 10.1371/journal.pntd.0003002. eCollection 2014 Aug.

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Association between Strongyloides stercoralis infection and cortisol secretion in alcoholic patients.

Silva ML, Inês Ede J, Souza AB, Dias VM, Guimarães CM, Menezes ER, Barbosa LG, Alves Mdel C, Teixeira MC, Soares NM.

Acta Trop. 2016 Feb;154:133-8. doi: 10.1016/j.actatropica.2015.11.010. Epub 2015 Nov 22.

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Select item 1650065839.


Hình 5

Successful transgenesis of the parasitic nematode Strongyloides stercoralis requires endogenous non-coding control elements.

Li X, Massey HC Jr, Nolan TJ, Schad GA, Kraus K, Sundaram M, Lok JB.

Int J Parasitol. 2006 May 31;36(6):671-9. Epub 2006 Feb 7.

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Strongyloides stercoralis transmission by kidney transplantation in two recipients from a common donor.

Roseman DA, Kabbani D, Kwah J, Bird D, Ingalls R, Gautam A, Nuhn M, Francis JM.

Am J Transplant. 2013 Sep;13(9):2483-6. doi: 10.1111/ajt.12390. Epub 2013 Aug 6.

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Pulmonary hyperinfection with Strongyloides stercoralis.

Huang MS, Hwang KP, Chiang PC, Hwang JJ.

J Formos Med Assoc. 1996 Jul;95(7):551-4.

PMID: 8840758 Similar articles

Discordances Between Serology and Culture for Strongyloides in an Ethiopian Adopted Child With Multiple Parasitic Infections: A Case Report.

Soriano-Arandes A, Sulleiro E, Zarzuela F, Ruiz E, Clavería I, Espasa M.

Medicine (Baltimore). 2016 Mar;95(10):e3040. doi: 10.1097/MD.0000000000003040.

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Epidemiology of Strongyloides stercoralis o­n Mekong islands in southern Laos.

Vonghachack Y, Sayasone S, Bouakhasith D, Taisayavong K, Akkavong K, Odermatt P.

Acta Trop. 2015 Jan;141(Pt B):289-94. doi: 10.1016/j.actatropica.2014.09.016. Epub 2014 Oct 5.

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A coproantigen diagnostic test for Strongyloides infection.

Sykes AM, McCarthy JS.

PLoS Negl Trop Dis. 2011 Feb 8;5(2):e955. doi: 10.1371/journal.pntd.0000955.

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Hyperinfection with Strongyloides stercoralis.

Romero-Cabello R, Villagroy Gómez J, Hernández González M, Romero Feregrino R.

BMJ Case Rep. 2012 Nov 30;2012. pii: bcr2012006819. doi: 10.1136/bcr-2012-006819.

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Prevalence of Strongyloides stercoralis and other intestinal parasitic infections among mentally retarded residents in central institution of southern Iran.

Shokri A, Sarasiabi KS, Teshnizi SH, Mahmoodi H.

Asian Pac J Trop Biomed. 2012 Feb;2(2):88-91. doi: 10.1016/S2221-1691(11)60198-6.

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Hình 6

Increased sensitivity of routine laboratory detection of Strongyloides stercoralis and hookworm by agar-plate culture.

Jongwutiwes S, Charoenkorn M, Sitthichareonchai P, Akaraborvorn P, Putaporntip C.

Trans R Soc Trop Med Hyg. 1999 Jul-Aug;93(4):398-400.

PMID: 10674087 Similar articles

A Schistosoma haematobium-specific real-time PCR for diagnosis of urogenital schistosomiasis in serum samples of international travelers and migrants.

Cnops L, Soentjens P, Clerinx J, Van Esbroeck M.

PLoS Negl Trop Dis. 2013 Aug 29;7(8):e2413. doi: 10.1371/journal.pntd.0002413. eCollection 2013.

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A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye.

Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.

Am J Trop Med Hyg. 2014 Feb;90(2):306-11. doi: 10.4269/ajtmh.13-0583. Epub 2013 Dec 9.

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Disseminated Strongyloides stercoralis Infection in an AIDS Patient: The Role of Suppressive Therapy.

Levi GC, Kallás EG, Ramos Moreira Leite K.

Braz J Infect Dis. 1997 Mar;1(1):48-51.

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[An overview of Strongyloides stercoralis and its infections].

Ardiç N.

Mikrobiyol Bul. 2009 Jan;43(1):169-77. Review. Turkish.

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Real-time PCR for Strongyloides stercoralis-associated meningitis.

Nadir E, Grossman T, Ciobotaro P, Attali M, Barkan D, Bardenstein R, Zimhony O.

Diagn Microbiol Infect Dis. 2016 Mar;84(3):197-9. doi: 10.1016/j.diagmicrobio.2015.11.015. Epub 2015 Nov 17.

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Occurrence of and risk factors for Strongyloides stercoralis infection in South-East Asia.

Schär F, Giardina F, Khieu V, Muth S, Vounatsou P, Marti H, Odermatt P.

Acta Trop. 2016 Jul;159:227-38. doi: 10.1016/j.actatropica.2015.03.008. Epub 2015 Mar 17. Review.

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Adult female of Strongyloides stercoralis in respiratory secretions.

Bava BA, Cecilia D, Alcides T.

Asian Pac J Trop Biomed. 2013 Apr;3(4):311-3. doi: 10.1016/S2221-1691(13)60069-6.

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Transmammary transmission of Strongyloides stercoralis in dogs.

Shoop WL, Michael BF, Eary CH, Haines HW.

J Parasitol. 2002 Jun;88(3):536-9.

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Hình 7

Strongyloidiasis: challenges in diagnosis and management in non-endemic Kuwait.

Hira PR, Al-Ali F, Shweiki HM, Abdella NA, Johny M, Francis I, Iqbal J, Thompson R, Nevar F.

Ann Trop Med Parasitol. 2004 Apr;98(3):261-70.

PMID: 1511997 Similar articles

Evaluation of strongyloidiasis in kennel dogs and keepers by parasitological and serological assays.

Gonçalves AL, Machado GA, Gonçalves-Pires MR, Ferreira-Júnior A, Silva DA, Costa-Cruz JM.

Vet Parasitol. 2007 Jun 20;147(1-2):132-9. Epub 2007 Apr 19.

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A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny.

Nagayasu E, Aung MPPTHH, Hortiwakul T, Hino A, Tanaka T, Higashiarakawa M, Olia A, Taniguchi T, Win SMT, Ohashi I, Odongo-Aginya EI, Aye KM, Mon M, Win KK, Ota K, Torisu Y, Panthuwong S, Kimura E, Palacpac NMQ, Kikuchi T, Hirata T, Torisu S, Hisaeda H, Horii T, Fujita J, Htike WW, Maruyama H.

Sci Rep. 2017 Jul 7;7(1):4844. doi: 10.1038/s41598-017-05049-x.

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Strongyloides stercoralis: The Most Prevalent Parasitic Cause of Eosinophilia in Gilan Province, Northern Iran.

Ashrafi K, Tahbaz A, Rahmati B.

Iran J Parasitol. 2010 Sep;5(3):40-7.

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Exploring features and function of Ss-riok-3, an enigmatic kinase gene from Strongyloides stercoralis.

Yuan W, Liu Y, Lok JB, Stoltzfus JD, Gasser RB, Lei W, Fang R, Zhao J, Hu M.

Parasit Vectors. 2014 Dec 5;7:561. doi: 10.1186/s13071-014-0561-z.

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Hình 8

Immunofluorescence assay for diagnosis of strongyloidiasis in immunocompromised patients.

Gottardi M, Paula FM, Corral MA, Meisel DM, Costa SF, Abdala E, Pierrotti LC, Yamashiro J, Chieffi PP, Gryschek RC.

Infect Dis (Lond). 2015 Aug;47(8):550-4. doi: 10.3109/23744235.2015.1028096. Epub 2015 Apr 2.

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Strongyloides stercoralis infection presenting as generalized prurigo nodularis and lichen simplex chronicus.

Jacob CI, Patten SF.

J Am Acad Dermatol. 1999 Aug;41(2 Pt 2):357-61.

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Specific IgA and IgG antibodies in paired serum and breast milk samples in human strongyloidiasis.

Mota-Ferreira DM, Gonçalves-Pires Mdo R, Júnior AF, Sopelete MC, Abdallah VO, Costa-Cruz JM.

Acta Trop. 2009 Feb;109(2):103-7. doi: 10.1016/j.actatropica.2008.09.023. Epub 2008 Oct 21.

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Improved diagnosis of Strongyloides stercoralis using recombinant antigen-based serologies in a community-wide study in northern Argentina.

Krolewiecki AJ, Ramanathan R, Fink V, McAuliffe I, Cajal SP, Won K, Juarez M, Di Paolo A, Tapia L, Acosta N, Lee R, Lammie P, Abraham D, Nutman TB.

Clin Vaccine Immunol. 2010 Oct;17(10):1624-30. doi: 10.1128/CVI.00259-10. Epub 2010 Aug 25.

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Hình 9

Comparison of three immunoassays for detection of antibodies to Strongyloides stercoralis.

Anderson NW, Klein DM, Dornink SM, Jespersen DJ, Kubofcik J, Nutman TB, Merrigan SD, Couturier MR, Theel ES.

Clin Vaccine Immunol. 2014 May;21(5):732-6. doi: 10.1128/CVI.00041-14. Epub 2014 Mar 19.

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