The mosquito dissection technique can also determine the developmental stages of mosquitoes based on specific criteria when observing the egg strings. To determine these stages, it is crucial to identify the physiological condition of the collected female mosquitoes. Previously, the physiological condition of mosquitoes was determined using the sella index, ranging from sella I to sella VII.
Surgeries on humans, livestock, and poultry are commonly discussed, but within the specialized field of medical entomology, an often-employed technique in regions where malaria is prevalent or during outbreaks involves mosquito dissection. This practice aims to study various physiological traits of mosquitoes, such as determining their mating status, parity status, frequency of egg laying, identifying different developmental stages of mosquito eggs, and assessing the mosquitoes' role in disease transmission.
Central Vietnam is the hardest hit region by malaria in the country for many decades with high rates of malaria morbidity and mortality. This region is characterized by its geographical location with long borderlines adjacent to Laos and Cambodia, along with the presence of nature reservations and national forests. This region is also inhabited by many ethnic minorities whose livelihoods are closely linked to logging and forest products exploitation.
This study is the first to apply MALDI-TOF MS to the identification of field-caught mosquitoes in Vietnam, where the burden of disease transmitted by these vectors is high. It confirms that misidentification of mosquitoesby morphology can be prevented with MALDI-TOF MS, provided that the reference spectrum of that mosquito species is present in the MALDI-TOF MS database.
Backgrounds: Fascioliasis is a disease of the hepatobiliary system, caused by Fasciola spp that are increasing and threating of public health in the tropic areas (Africa and Asia), including of Central part of Vietnam. WHO estimates that at least 2.4 million people are infected in more than 70 countries worldwide, with several million at risk.No continent is free from fascioliasis, and it is likely that where animal cases are reported, human cases also exist. This study carried out to evaluate several particular clinical aspects in the vulnerable groups with fascioliasis.
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