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 Thư viện điện tử Công trình nghiên cứu về Ký sinh trùng
Phần 2: Nghiên cứu phân tích phân tử về định loài giun lươn gây bệnh ở người

Giun lươn Strongyloides spp. gây bệnh cho người có thể xuất phát từ 3 loài trong giống Strongyloides, sự phân bố ưu thế từng loài tùy thuộc vào vùng địa lý, sự di chuyển dân di biến động và nhóm dân tỵ nạn, hay các bệnh viện tâm thần và nơi điều trị các bệnh nhân suy giảm miễn dịch đã phân tích cho thấy tỷ lệ từng loài của mỗi nơi cũng khác nhau.

Trên thế giới cũng như tại Việt Nam, một số loài giun lươn đã được phân tích, song chưa được bài bản và quy mô rộng trên các quần thể ở các vùng địa lý khác nhau. Dưới đây là một số nghiên cứu trên thế giới và Việt Nam đã định loài, phân tích phả hệ, di truyền của loài giun lươn bằng nhiều phương pháp và kỹ thuật khác nhau như PCR, Real-time PCR, Nested PCR, LAMP, DNA isolation, RFLP . Xin chia sẻ cùng đồng nghiệp.

Screening of immunocompromised patients at risk of strongyloidiasis in western Turkey using ELISA and real-time PCR.

Erdem Kivrak E, Atalay Şahar E, Can H, Döşkaya M, Yilmaz M, Pullukçu H, Caner A, Töz H, Gürüz AY, Işikgöz Taşbakan M.

Turk J Med Sci. 2017 Jun 12;47(3):897-901. doi: 10.3906/sag-1605-19.

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Evaluation of real-time PCR for Strongyloides stercoralis and hookworm as diagnostic tool in asymptomatic schoolchildren in Cambodia.

Schär F, Odermatt P, Khieu V, Panning M, Duong S, Muth S, Marti H, Kramme S.

Acta Trop. 2013 May;126(2):89-92. doi: 10.1016/j.actatropica.2012.12.012. Epub 2013 Jan 5.

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High prevalence and spatial distribution of Strongyloides stercoralis in rural Cambodia.

Khieu V, Schär F, Forrer A, Hattendorf J, Marti H, Duong S, Vounatsou P, Muth S, Odermatt P.

PLoS Negl Trop Dis. 2014 Jun 12;8(6):e2854. doi: 10.1371/journal.pntd.0002854. eCollection 2014 Jun.

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Strongyloides stercoralis infection and re-infection in a cohort of children in Cambodia.

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Hình 2

Diagnosing Polyparasitism in a High-Prevalence Setting in Beira, Mozambique: Detection of Intestinal Parasites in Fecal Samples by Microscopy and Real-Time PCR.

Meurs L, Polderman AM, Vinkeles Melchers NV, Brienen EA, Verweij JJ, Groosjohan B, Mendes F, Mechendura M, Hepp DH, Langenberg MC, Edelenbosch R, Polman K, van Lieshout L.

PLoS Negl Trop Dis. 2017 Jan 23;11(1):e0005310. doi: 10.1371/journal.pntd.0005310. eCollection 2017 Jan.

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Strongyloides stercoralis and other enteroparasites in children at Uberlândia city, state of Minas Gerais, Brazil.

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Syndrome of Inappropriate Anti-Diuretic Hormone Secretion Secondary to Strongyloides stercoralis Infection in an Allogeneic Stem Cell Transplant Patient: A Case Report and Literature Review.

Khushman M, Morris MI, Diaz L, Goodman M, Pereira D, Fuller K, Garcia-Buitrago M, Moshiree B, Zelaya S, Nayer A, Benjamin CL, Komanduri KV.

Transplant Proc. 2017 Mar;49(2):373-377. doi: 10.1016/j.transproceed.2016.12.012. Review.

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Detection of hookworm and hookworm-like larvae in human fecocultures in Suriname.

Jozefzoon LM, Oostburg BF.

Am J Trop Med Hyg. 1994 Oct;51(4):501-5.

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Predictive markers for development of strongyloidiasis in patients infected with both Strongyloides stercoralis and HTLV-1.

Satoh M, Kiyuna S, Shiroma Y, Toma H, Kokaze A, Sato Y.

Clin Exp Immunol. 2003 Sep;133(3):391-6.

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Parasitological versus molecular diagnosis of strongyloidiasis in serial stool samples: how many?

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J Helminthol. 2018 Jan;92(1):12-16. doi: 10.1017/S0022149X17000050. Epub 2017 Jan 23.

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Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis.

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PLoS Negl Trop Dis. 2018 Feb 9;12(2):e0006229. doi: 10.1371/journal.pntd.0006229. eCollection 2018 Feb.

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Hình 4

Detection of piggyBac-mediated Transposition by Splinkerette PCR in Transgenic Lines of Strongyloides ratti.

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Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis.

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Incidental Detection of Strongyloides stercoralis in a Routine Cervicovaginal Smear.

Kumar SP, Mallya V.

J Cytol. 2017 Jan-Mar;34(1):69-70. doi: 10.4103/0970-9371.197627. No abstract available.

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