Phần 2: Nghiên cứu sinh học phân tử trên các loại sán lá, đơn bào và mối liên quan đến ung thư cơ quan ở người
Trong bối cảnh biến đổi khí hậu toàn cầu (Global Climate change), nhiều bệnh truyền nhiễm đang nổi và tái nổi có xu hướng tăng lên đáng kể và đe dọa tử vong cũng như gia tăng gánh nặng bệnh tật toàn cầu. Trong đó một số bệnh ký sinh trùng và đơn bào có liên quan có ý nghĩa với một số bệnh ung thư như sán lá gan nhỏ Opisthorchis viverrini , Clonorchis sinensis liên quan đến ung thư biểu mô đường mật (cholangiocarcinoma), hay sán máng Schitosoma haematobium liên quan ung thư bàng quan tiết niệu, hay Cryptosporidium parvum có liên quan đến ung thư đại trực tràng (colorectal cancer).
Bên cạnh đó, việc xác định loài các giun sán về mặt hình thái học và sinh học phân tử phần nào làm rõ về phả hệ di truyền của các loài ký sinh trùng gây bệnh ở người. Các nghiên cứu sau đây làm rõ các vấn đề mà gần đây các nhà khoa học đang quan tâm làm rõ.
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TaqMan real-time PCR assay for specific detection of Opisthorchis viverrini DNA in Thai patients with hepatocellular carcinoma and cholangiocarcinoma.
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Genome-wide characterization of microsatellites and marker development in the carcinogenic liver fluke Clonorchis sinensis.
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Potassium permanganate staining for differentiation the surface morphology of Opisthorchis viverrini, Haplorchis taichui and Phaneropsolus bonnei eggs.
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Methylene blue staining method for identification of Opisthorchis viverrini egg.
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Recurrent emergence of structural variants of LTR retrotransposon CsRn1 evolving novel expression strategy and their selective expansion in a carcinogenic liver fluke, Clonorchis sinensis.
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Human infections with liver and minute intestinal flukes in Guangxi, China: analysis by DNA sequencing, ultrasonography, and immunoaffinity chromatography.
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Discovery of Opisthorchis lobatus (Trematoda: Opisthorchiidae): a new record of small liver flukes in the Greater Mekong Sub-region.
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Detection of Opisthorchis viverrini in human stool specimens by PCR.
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Molecular characterization of a tetraspanin from the human liver fluke, Opisthorchis viverrini.
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Molecular genetic profiles among individual Clonorchis sinensis adults collected from cats in two geographic regions of China revealed by RAPD and MGE-PCR methods.
Lai DH, Wang QP, Chen W, Cai LS, Wu ZD, Zhu XQ, Lun ZR.
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A novel, multiplex, real-time PCR-based approach for the detection of the commonly occurring pathogenic fungi and bacteria.
Horváth Á, Pető Z, Urbán E, Vágvölgyi C, Somogyvári F.
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Kummalue T, Chuphrom A, Sukpanichanant S, Pongpruttipan T, Sukpanichanant S.
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Genetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini from Savannakhet, Lao PDR by multilocus enzyme electrophoresis.
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Molecular changes in Opisthorchis viverrini (Southeast Asian liver fluke) during the transition from the juvenile to the adult stage.
Jex AR, Young ND, Sripa J, Hall RS, Scheerlinck JP, Laha T, Sripa B, Gasser RB.
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[Identification of Clonorchis sinensis metacercariae based on PCR targeting ribosomal DNA ITS regions and COX1 gene].
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Liver fluke infection and cholangiocarcinoma: a review.
Zheng S, Zhu Y, Zhao Z, Wu Z, Okanurak K, Lv Z.
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Dao TH, Nguyen TG, Victor B, Gabriël S, Dorny P.
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Characterization of the antioxidant enzyme, thioredoxin peroxidase, from the carcinogenic human liver fluke, Opisthorchis viverrini.
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Microscopic identification of possible Clonorchis/Opisthorchis infection in two Ghanaian women with undiagnosed abdominal discomfort: two case reports.
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Parasite-Associated Cancers (Blood Flukes/Liver Flukes).
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